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Related Experiment Video

Updated: May 9, 2026

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Published on: February 25, 2022

Bringing optogenetics to the synapse.

Andreas Reiner¹, Joshua Levitz, Ehud Isacoff

¹Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute, 271 Life Sciences Addition, University of California, Berkeley, Berkeley, CA 94720, USA.

|July 30, 2013

Summary

This summary is machine-generated.

Researchers developed InSynC, a novel optogenetic method using chromophore-assisted light inactivation (CALI). This technique effectively reduces presynaptic neurotransmitter release proteins, impacting synaptic transmission in targeted areas.

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Published on: March 14, 2018

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

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Last Updated: May 9, 2026

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Published on: February 25, 2022

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

Published on: March 14, 2018

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Published on: January 15, 2015

Area of Science:

Neuroscience
Optogenetics
Molecular Biology

Background:

Presynaptic proteins like VAMP2 and synaptophysin are crucial for neurotransmitter release.
Precisely controlling synaptic transmission is essential for understanding neural circuits.

Purpose of the Study:

To introduce InSynC, a new optogenetic tool for targeted inactivation of presynaptic proteins.
To demonstrate the efficacy of InSynC in reducing synaptic transmission in specific regions.

Main Methods:

Utilized chromophore-assisted light inactivation (CALI) as the core mechanism.
Applied the InSynC method to inactivate VAMP2 and synaptophysin.
Validated the technique in both in vitro and in vivo experimental settings.

Main Results:

Successfully inactivated presynaptic neurotransmitter release proteins VAMP2 and synaptophysin.
Demonstrated selective reduction of synaptic transmission in illuminated areas.
Showcased the applicability of InSynC in diverse experimental conditions.

Conclusions:

InSynC provides a powerful and spatially precise optogenetic approach for studying synaptic function.
This method allows for targeted manipulation of neurotransmitter release, offering new avenues for neuroscience research.