Direct electrospray ionization mass spectrometric profiling of real-world samples via a solid sampling probe

Area of Science:

• Analytical Chemistry
• Biochemistry
• Mass Spectrometry

Background:

• Traditional biochemical analysis often requires extensive sample preparation, increasing time and cost.
• Direct analysis methods are needed for rapid profiling of complex real-world samples.
• Existing direct sampling techniques may lack efficiency or versatility.

Purpose of the Study:

• To develop a novel, simplified direct analysis strategy for rapid mass spectrometric profiling.
• To enable direct analysis of biochemicals in real-world samples without pretreatment.
• To create a cost-effective and disposable tool for biochemical analysis.

Main Methods:

• A direct sampling probe (DSP) was developed using a chemically modified acupuncture needle.
• The DSP was designed to enhance surface area and hydrophilicity for biofluid attachment.
• Electrospray ionization was achieved using high DC voltage and solvent vapor condensation.

Main Results:

• The DSP enabled rapid mass spectrometric profiling of biochemicals directly from real-world samples.
• The method eliminated the need for sample pretreatments.
• The modified acupuncture needle facilitated efficient analyte dissolution and electrospraying.

Conclusions:

• The novel DSP offers a simple, versatile, and cost-effective solution for direct biochemical analysis.
• This method presents a competitive alternative for rapid analysis of complex biological samples.
• The disposable nature of the probe enhances its practicality for routine applications.