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In vivo Characterization of Endocrine Disrupting Chemical Effects via Thyroid Hormone Action Indicator Mouse
Published on: October 6, 2023
Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different
Miriane de Oliveira1, Renata de Azevedo Melo Luvizotto, Regiane Marques Castro Olimpio
1Department of Internal Clinic, Botucatu Medicine School, São Paulo State University, Botucatu, SP, Brazil. miriane.deoliveira@yahoo.com.br
Objective:
To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TRα and TRβ, at different times.
Materials And Methods:
3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TRα and TRβ mRNA was detected using real-time polymerase chain reaction.
Results:
F increased TRβ mRNA levels at 0.5h. After 1h, TRα levels increased with F and SI and TRβ levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TRα and TRβ levels were similar to those of C group.
Conclusions:
T3 action with F began at 1h for TRα and at 0.5h for TRβ. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.
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