Related Experiment Video

Updated: May 9, 2026

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
08:32

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Published on: December 13, 2013

[Advantages and disadvantages of zink-ethanol-formaldehyde as a fixative for immunocytochemistry and confocal laser

D E Korzhevskiĭ, E G Sukhorukova, E G Gilerovich

    Morfologiia (Saint Petersburg, Russia)
    |August 1, 2013
    PubMed

    More Related Videos

    Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections09:11

    Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

    Published on: November 14, 2016

    Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods08:37

    Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

    Published on: June 25, 2019

    Related Concept Videos

    Fixation and Sectioning01:03

    Fixation and Sectioning

    Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
    The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
    Confocal Fluorescence Microscopy01:16

    Confocal Fluorescence Microscopy

    Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
    Immunogold Electron Microscopy01:20

    Immunogold Electron Microscopy

    Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
    Three-Dimensional Microscopy in Microbiology01:28

    Three-Dimensional Microscopy in Microbiology

    Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...