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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: May 9, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Note: multi-confocal fluorescence correlation spectroscopy in living cells using a complementary metal oxide

M Kloster-Landsberg1, D Tyndall, I Wang

  • 1University of Grenoble 1/CNRS, LIPhy UMR 5588, F-38041 Grenoble, France.

The Review of Scientific Instruments
|August 2, 2013
PubMed
Summary

Researchers developed a new multi-confocal system for simultaneous fluorescence correlation spectroscopy. This technique measures molecular dynamics in multiple locations within living cells, advancing cellular analysis.

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Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
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Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

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Related Experiment Videos

Last Updated: May 9, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
06:51

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

Area of Science:

  • Biophysics
  • Cell Biology
  • Optical Spectroscopy

Background:

  • Living cells are dynamic and heterogeneous environments.
  • Measuring molecular concentration and dynamics simultaneously across multiple cellular locations is crucial for understanding cellular processes.
  • Existing techniques may lack the throughput or spatial resolution for comprehensive analysis.

Purpose of the Study:

  • To develop and demonstrate a novel multi-confocal setup for simultaneous fluorescence correlation spectroscopy (FCS) measurements.
  • To enable high-throughput analysis of molecular dynamics in living cells.
  • To overcome limitations of single-point measurements in heterogeneous cellular environments.

Main Methods:

  • A multi-confocal setup utilizing a spatial light modulator (SLM) to focus laser spots.
  • Data acquisition using a monolithic 32 × 32 single-photon avalanche photodiode (SPAD) array.
  • Development of a post-processing method to correct for cross-talk between adjacent measurement spots.

Main Results:

  • Simultaneous FCS measurements were successfully performed at nine distinct locations.
  • The system demonstrated the ability to measure the diffusion dynamics of enhanced Green Fluorescent Protein (eGFP) in living cells.
  • The proposed post-processing method effectively mitigated cross-talk artifacts.

Conclusions:

  • The developed multi-confocal SPAD array system provides a powerful tool for simultaneous, multi-point molecular dynamics measurements in living cells.
  • This technology enhances the study of cellular heterogeneity and dynamics.
  • The system offers a significant advancement for high-throughput biophysical analysis within biological systems.