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D(-)-N-Methylglucamine buffer for pH 8.5 to 10.5.

V Chromy, V Kulhánek, J Fischer

    Clinical Chemistry
    |February 1, 1978
    PubMed
    Summary
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    A novel amine buffer system using D(-)-N-methylglucamine offers reliable pH control between 8.5 and 10.5. This highly soluble buffer is suitable for biochemical applications and general pH management.

    Area of Science:

    • Biochemistry
    • Analytical Chemistry

    Background:

    • Accurate pH control is crucial in various scientific disciplines.
    • Existing buffer systems may have limitations in specific pH ranges or stability.
    • D(-)-N-methylglucamine is a readily available, highly purified compound.

    Purpose of the Study:

    • To introduce and characterize a new amine buffer system.
    • To determine the key physicochemical properties of the D(-)-N-methylglucamine buffer.
    • To assess the suitability of this buffer for biochemical and general pH control applications.

    Main Methods:

    • Preparation of buffer solutions using D(-)-N-methylglucamine and its hydrochloride.
    • Determination of thermodynamic pKa, buffer capacity, dilution value, temperature coefficient, and heat of ionization.

    Related Experiment Videos

  • Evaluation of buffer solubility and stability.

    • Main Results:

    • The D(-)-N-methylglucamine buffer system operates effectively in the pH range of 8.5 to 10.5.
    • Key constants determined include: thermodynamic pKa = 9.52 (25°C), buffer capacity = 0.055, dilution value pH₁/₂ = -0.02, temperature coefficient dpKa/dT = 0.023, and heat of ionization ΔH° = 36.93 kJ mol⁻¹.
    • The buffer exhibits high water solubility and can be prepared as stable solutions or solid mixtures.

    Conclusions:

    • D(-)-N-methylglucamine and its hydrochloride form a robust and versatile buffer system.
    • The characterized physicochemical properties make it suitable for precise pH control in biochemistry.
    • This new buffer offers a valuable option for applications requiring stable pH in the 8.5-10.5 range.