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An Injectable and Drug-loaded Supramolecular Hydrogel for Local Catheter Injection into the Pig Heart
Published on: June 7, 2015
Immunotherapy with injectable hydrogels to treat obstructive nephropathy.
Danielle E Soranno1, Hoang D Lu, Heather M Weber
1Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, Pennsylvania.
Journal of Biomedical Materials Research. Part A
|August 6, 2013
Summary
Injectable hydrogels deliver interleukin-10 (IL-10) to reduce inflammation and fibrosis in chronic kidney disease. This targeted therapy effectively decreased macrophage infiltration and apoptosis, supporting hydrogel use for kidney disease treatment.
Area of Science:
- Biomaterials Science
- Nephrology
- Regenerative Medicine
Background:
- Hydrogels are emerging as injectable drug delivery systems.
- Chronic kidney disease (CKD) is characterized by inflammation and fibrosis.
- Local delivery of therapeutics can improve treatment efficacy.
Purpose of the Study:
- To develop and evaluate self-assembling, injectable hydrogels for local delivery of interleukin-10 (IL-10).
- To assess the hydrogel's efficacy in mitigating inflammation and fibrosis in a mouse model of CKD.
Main Methods:
- Dock-and-Lock hydrogels were synthesized for IL-10 encapsulation.
- A unilateral ureteral obstruction (UUO) mouse model was used.
- Hydrogel/IL-10 was injected, and outcomes were assessed via immunohistochemistry and trichrome staining for inflammation, apoptosis, and fibrosis.
Main Results:
- In vitro, hydrogels degraded within days, releasing IL-10.
- In vivo, hydrogels persisted in the kidney for up to 30 days.
- Hydrogel-delivered IL-10 significantly reduced macrophage infiltration and apoptosis at 21 and 35 days post-UUO compared to controls and IL-10 alone. Fibrosis was reduced in all treatment groups by day 35.
Conclusions:
- Injectable hydrogels provide sustained local delivery of IL-10.
- Hydrogel-based IL-10 delivery is a promising strategy for treating inflammation and fibrosis in CKD.
- This approach warrants further investigation for clinical application in kidney disease.
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