Sysmex UF-1000i performance for screening yeasts in urine

Area of Science:

• Clinical Microbiology
• Diagnostic Technology
• Urinalysis

Background:

• Candiduria, or yeast in urine, requires accurate and timely detection for appropriate patient management.
• Traditional urine culture methods can be time-consuming, delaying diagnosis and treatment.

Purpose of the Study:

• To evaluate the diagnostic performance of the Sysmex UF-1000i automated urine analyzer for detecting yeasts in clinical urine samples.
• To determine an optimal cutoff for yeast-like cells (YLCs)/μL for reliable candiduria screening.

Main Methods:

• Screening of 22,132 clinical urine samples using the Sysmex UF-1000i.
• Analysis of simulated urine samples with known Candida albicans concentrations.
• Calculation of sensitivity, specificity, and predictive values at a defined YLCs/μL cutoff.

Main Results:

• A cutoff of 50 YLCs/μL demonstrated high sensitivity (87.3%-95.4%) and specificity (97%) for detecting candiduria ≥10,000 CFU/mL.
• A linear correlation was observed between dilution factor and UF-1000i detection in simulated samples.
• The system reliably ruled out candiduria ≥10,000 CFU/mL.

Conclusions:

• The Sysmex UF-1000i system is a valuable tool for screening candiduria, offering rapid and reliable results.
• An appropriate YLCs/μL cutoff, considering local prevalence, can optimize its use for candiduria detection.
• This automated system can significantly improve the efficiency of urine sample screening in microbiology laboratories.