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Programming microbes using pulse width modulation of optical signals.

Eric A Davidson1, Amar S Basu, Travis S Bayer

  • 1Centre for Synthetic Biology and Innovation and Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, UK.

Journal of Molecular Biology
|August 10, 2013
PubMed
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This study shows bacterial light-responsive systems act as low-pass filters. This filtering behavior enables precise control of gene expression using pulse width modulation (PWM) for synthetic biology applications.

Area of Science:

  • Synthetic biology
  • Systems biology
  • Bacterial genetics

Background:

  • Cellular signaling pathways encode information in temporal dynamics.
  • Pathway architecture influences signal propagation in time and space.
  • Synthetic biologists can leverage pathway properties for precise control of cellular functions.

Purpose of the Study:

  • To characterize the response of a bacterial light-responsive system to oscillating signals.
  • To demonstrate the utility of pulse width modulation (PWM) for controlling gene expression in bacteria.
  • To extend the application of bacterial optogenetic control.

Main Methods:

  • Characterization of a bacterial light-responsive two-component system's response to varying frequencies of oscillating signals.
Keywords:
LEDPWMlight emitting diodelow-pass filteroptogeneticspulse width modulationsignal transduction

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  • Application of pulse width modulation (PWM) strategy for gene expression control.
  • Manipulation of a metabolic enzyme to control microbial physiology.

    • Main Results:

    • The bacterial system functions as a low-pass filter, responding to low-frequency but not high-frequency oscillations.
    • Pulse width modulation (PWM) effectively controls gene expression levels and temporal dynamics.
    • PWM strategy allows fine-tuning of expression and control of microbial physiology.

    Conclusions:

    • Bacterial light-responsive systems exhibit low-pass filtering properties.
    • Pulse width modulation (PWM) is a viable strategy for precise optogenetic control in bacteria.
    • This approach enhances the utility of synthetic biology for programming cellular behavior and physiology.