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Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
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Multicellular aggregates: a model system for tissue rheology.

Tomita Vasilica Stirbat1, Sham Tlili, Thibault Houver

  • 1Institut Lumière Matière, UMR5306 Université de Lyon 1-CNRS, Université de Lyon, 69622 Villeurbanne cedex, France.

The European Physical Journal. E, Soft Matter
|August 10, 2013
PubMed
Summary
This summary is machine-generated.

Cells exhibit a softening behavior under stress, revealing a critical yield point. This finding highlights the importance of viewing cells as complex fluids with time-varying properties influenced by stress history.

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Area of Science:

  • Biophysics
  • Developmental Biology
  • Cell Mechanics

Background:

  • Morphogenesis relies on cell flows, where tissue mechanical response is tied to viscosity.
  • Understanding this response independent of genetic changes is crucial for developmental studies.

Purpose of the Study:

  • To investigate the rheological properties of multicellular aggregates as tissue models.
  • To decouple mechanical responses from complex genetic alterations during development.

Main Methods:

  • Rheometry experiments were conducted on multicellular aggregates.
  • A sensitive technique was employed to measure cell behavior under stress.

Main Results:

  • Observed cell softening behavior under applied stresses.
  • Identified a yield point at strains above 12%, beyond which a creep regime occurs.
  • Proposed a dynamic cytoskeleton model (parallel springs breaking/reattaching) to explain rheological curves.

Conclusions:

  • Cells behave as complex fluids whose properties are history-dependent.
  • The proposed cytoskeleton model effectively reproduces observed cell strain behaviors.
  • Emphasizes the significance of considering cellular viscoelasticity in developmental processes.