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Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms
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Published on: January 20, 2022

Molecular diagnostics and parasitic disease.

Shawn Vasoo1, Bobbi S Pritt

  • 1Department of Laboratory Medicine and Pathology, Division of Clinical Microbiology, Mayo Clinic, 200 1st Street Southwest, Rochester, MN 55905, USA.

Clinics in Laboratory Medicine
|August 13, 2013
PubMed
Summary

Molecular parasitology diagnostics are advancing with new commercial systems and promising field-ready methods for diseases like malaria. This review covers current techniques and their applications in endemic parasitic infections.

Keywords:
AmebiasisBabesiosisLeishmaniasisMalariaNAATPCRTrichomonasTrypanosomiasis

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Published on: February 28, 2015

Area of Science:

  • Microbiology diagnostics
  • Molecular parasitology

Background:

  • Molecular parasitology is an emerging field in microbiology.
  • Current diagnostic assays often rely on nonstandardized, laboratory-developed methods.
  • Commercial systems are slowly being introduced, with some methods showing field potential.

Purpose of the Study:

  • To review available molecular diagnostic techniques for parasitic diseases.
  • To discuss the applications of these techniques in major parasitic infections.
  • To highlight emerging methodologies for endemic settings.

Main Methods:

  • Review of current literature on molecular parasitology assays.
  • Analysis of commercial systems and laboratory-developed methods.
  • Assessment of techniques for specific parasitic diseases.

Main Results:

  • Several molecular diagnostic techniques are available for parasitic infections.
  • Commercial systems are emerging, though not yet widespread.
  • Promising field-applicable methodologies exist for endemic areas.

Conclusions:

  • Molecular parasitology offers advanced diagnostic capabilities.
  • Standardization and accessibility of methods are key challenges.
  • Further development is needed for widespread clinical and field application.