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Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

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Cell matrix remodeling ability shown by image spatial correlation.

Chi-Li Chiu¹, Michelle A Digman, Enrico Gratton

¹Department of Developmental and Cell Biology, University of California, Irvine, CA 92697, USA.

Journal of Biophysics (Hindawi Publishing Corporation : Online)

|August 13, 2013

Summary

This summary is machine-generated.

This study introduces a new quantitative method using image spatial correlation to measure extracellular matrix (ECM) remodeling by cells. The technique reveals distinct collagen remodeling abilities among different cell lines and drug treatments.

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Area of Science:

Biophysics
Cell Biology
Biomaterials Science

Background:

Extracellular matrix (ECM) remodeling is crucial in biological and pathological processes.
Existing methods lack quantitative measurement of ECM remodeling at the cellular scale.

Purpose of the Study:

To develop and apply a quantitative method for measuring cellular collagen remodeling.
To assess collagen remodeling by different cancer and fibroblast cell lines.
To evaluate the impact of specific drugs on cellular collagen remodeling.

Main Methods:

Application of image spatial correlation to collagen second harmonic generation (SHG) images.
Development of a statistical method using spatial correlation functions to quantify collagen density around cells.
Analysis of breast cancer cell lines (MDA-MB-231, MCF-7) and a fibroblast cell line (NIH/3T3).
Quantitative measurement of drug effects (Latrunculin B, Marimastat) on collagen remodeling.

Main Results:

The spatial correlation method successfully quantified collagen remodeling by different cell lines.
Distinct collagen compaction levels were observed, indicating varying remodeling abilities.
Latrunculin B and Marimastat treatments significantly decreased collagen compaction levels in MDA-MB-231 cells.

Conclusions:

The proposed spatial correlation method provides a quantitative approach to assess cellular ECM remodeling.
Cellular invasiveness correlates with distinct collagen remodeling capacities.
Pharmacological interventions can modulate cellular collagen remodeling capabilities.