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Related Experiment Video

Updated: May 8, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Resolution below the point spread function for diffuse optical imaging using fluorescence lifetime multiplexing.

William L Rice¹, Steven Hou, Anand T N Kumar

¹Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA.

|August 14, 2013

Summary

This summary is machine-generated.

Asymptotic lifetime-based fluorescence tomography precisely locates multiple targets, even when closely spaced. This advanced method uniquely utilizes decay-amplitude data for superior tomographic lifetime multiplexing.

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Last Updated: May 8, 2026

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Area of Science:

Biomedical Optics
Fluorescence Imaging
Tomography

Background:

Turbid media pose challenges for localizing multiple fluorescent targets due to diffuse light scattering.
Traditional fluorescence tomography methods struggle with resolving closely spaced targets below the diffuse point spread function.

Purpose of the Study:

To demonstrate the capability of asymptotic lifetime-based fluorescence tomography for resolving multiple closely spaced targets.
To highlight the advantages of decay-amplitude-based approaches over continuous wave or time-gated methods.

Main Methods:

Utilized a complete diagonalization of the time domain forward problem in the asymptotic limit.
Developed a lifetime-based fluorescence tomography approach.

Main Results:

Successfully localized multiple fluorescence targets separated below the diffuse point spread function.
Demonstrated that continuous wave and time-gated fluorescence tomography methods fail to achieve this separation.

Conclusions:

Asymptotic lifetime-based fluorescence tomography offers a unique advantage for resolving multiple targets in turbid media.
Decay-amplitude-based analysis of time-domain data is crucial for effective tomographic lifetime multiplexing.