Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High-speed hyperspectral single-pixel microscopy via line-scan detection with data fusion-based enhanced resolution.

Communications engineering·2026

Same author

Beam shaping techniques for pulsed laser ablation in liquids: Unlocking tunable control of nanoparticle synthesis in liquids.

Beilstein journal of nanotechnology·2026

Same author

Glomeruli detection and classification in histopathological images using deep learning semantic segmentation.

BMC medical imaging·2026

Same author

Single-pixel microscopy with enhanced lateral resolution.

Optics express·2025

Same author

Hadamard based single-pixel microscopy using sensor-less adaptive optics supported by multi-actuator adaptive lens.

Nature communications·2025

Same author

Sub-pixel deep learning ghost imaging for defect inspection in large areas using blurred illumination.

Applied optics·2025

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 8, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Compressive holography with a single-pixel detector.

Pere Clemente¹, Vicente Durán, Enrique Tajahuerce

¹GROC UJI, Institut de Noves Tecnologies de la Imatge, Universitat Jaume I, Castelló, Spain.

|August 14, 2013

Summary

This summary is machine-generated.

This study introduces compressive phase-shifting holography, merging interferometry and single-pixel imaging for advanced digital holography. This technique reconstructs object details using fewer measurements, improving optical imaging efficiency.

More Related Videos

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Published on: September 16, 2022

Related Experiment Videos

Last Updated: May 8, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Published on: September 16, 2022

Area of Science:

Optics and Photonics
Digital Imaging
Interferometry

Background:

Digital holography traditionally requires high-resolution sensors.
Compressive sensing offers efficient data acquisition by reducing sampling requirements.
Phase-shifting interferometry is crucial for reconstructing complex object information.

Purpose of the Study:

To develop a novel framework for digital holography at optical wavelengths.
To integrate phase-shifting interferometry with single-pixel optical imaging and compressive sensing.
To demonstrate a method for resolving the complex amplitude of an object with reduced data.

Main Methods:

Utilizing Hadamard patterns and a liquid crystal spatial light modulator for diffraction field sampling.
Adapting single-pixel camera principles with a Mach-Zehnder interferometer for interferometric imaging.
Applying phase-shifting techniques and backward light propagation algorithms for complex amplitude reconstruction.

Main Results:

Successful development of a compressive phase-shifting holography framework.
Demonstration of resolving the complex amplitude of an object.
Proof-of-concept experiment successfully evaluated the phase distribution of an ophthalmic lens.

Conclusions:

Compressive phase-shifting holography provides an efficient alternative to traditional digital holography.
The developed framework enables high-fidelity reconstruction with reduced data acquisition.
This technique has potential applications in optical metrology and imaging systems.