Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amelioration of neurogenic erectile dysfunction by ST36 electroacupuncture.

iScience·2026
Same author

Intra-Abdominal Umbilical Vein Varix With Postnatal Portal Vein Thrombosis: A Case Report.

Journal of clinical ultrasound : JCU·2026
Same author

How precise are mutation rate estimates? Comparison of different approaches to estimate de novo mutation rates.

Heredity·2026
Same author

iSymBase: an integrative functional-genomic platform for ecological exploration of insect symbionts.

ISME communications·2026
Same author

Quantitative evaluation of left atrial function via two-dimensional speckle-tracking echocardiography in patients with different grades of hypertension.

Quantitative imaging in medicine and surgery·2026
Same author

Basal Ganglia maturation in neonates using QSM and R2*: nucleus-specific, time-dependent trajectories and effects of prematurity.

NeuroImage·2026
Same journal

AQbD-Guided Chiral HPLC for Enantiomeric Impurity Analysis of Suzetrigine.

Chirality·2026
Same journal

Recent Progress in the Total Synthesis and Biosynthesis of Arborisidine.

Chirality·2026
Same journal

Advances in Chiral Separation Techniques for Calcium Channel Blockers: Analytical Strategies and Future Perspectives in Antihypertensive Drug Development (2015-2025).

Chirality·2026
Same journal

Practical Enantioselective Approach to 3-Amino-2-Hydroxy Acids and Application to the Synthesis of Natural Products.

Chirality·2026
Same journal

Chiral (Stereoselective) Drugs, Asymmetric Synthesis, and Racemic Resolution Methods.

Chirality·2026
Same journal

Capillary Chiral Separation of Agrochemicals: Complementary Insights From Molecular Modeling and NMR.

Chirality·2026
See all related articles

Related Experiment Video

Updated: May 8, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Improved chiral SFC screening for analytical method development.

Wes Schafer1, Tilak Chandrasekaran, Zainab Pirzada

  • 1Analytical Chemistry, Merck Research Laboratories, Rahway, New Jersey.

Chirality
|August 16, 2013
PubMed
Summary
This summary is machine-generated.

A new supercritical fluid chromatography (SFC) instrument enables faster and more universal chiral SFC method development. Its advanced controls and smaller particle columns significantly improve the process for researchers.

Keywords:
SFCchiral screeningfast analysismethod development

More Related Videos

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
09:44

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Published on: June 13, 2021

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Related Experiment Videos

Last Updated: May 8, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
09:44

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Published on: June 13, 2021

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Area of Science:

  • Analytical Chemistry
  • Chromatography Science

Background:

  • Supercritical Fluid Chromatography (SFC) is a powerful separation technique.
  • Automated method development is crucial for efficient chiral separations.

Purpose of the Study:

  • To evaluate a new supercritical fluid chromatography (SFC) instrument for automated chiral SFC method development.
  • To assess the instrument's performance compared to previous systems.

Main Methods:

  • Utilized a newly developed SFC instrument with enhanced gradient dwell volume and liquid flow control.
  • Employed shorter columns packed with smaller stationary phase particles.

Main Results:

  • The new instrument demonstrated significantly faster chiral SFC method development.
  • The system exhibited more universal applicability for chiral separations.
  • Improved gradient dwell volume and flow control were key factors.

Conclusions:

  • The evaluated SFC instrument represents a significant advancement in automated chiral method development.
  • The combination of instrument features and column technology enhances efficiency and scope.
  • This technology offers a more streamlined approach for chiral analysis.