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Hydrocortisone effect on hyaluronate synthesis in a self-assembled human dermal equivalent
Madhura Deshpande1, Suzanne Papp2, Lana Schaffer2
1Department of Pharmaceutical Sciences, Bouvé College of Health Sciences, Northeastern University, Boston, MA, USA.
Journal of Tissue Engineering and Regenerative Medicine
|August 20, 2013
Summary
Hydrocortisone affects hyaluronic acid (HA) synthesis in human dermal matrix. Low doses enhance HA production, while high doses inhibit it by impacting early glycolytic pathways and gene expression.
Area of Science:
- Biochemistry
- Dermatology
- Molecular Biology
Background:
- Human dermal matrix is a self-assembled dermal equivalent rich in hyaluronic acid (HA).
- Hydrocortisone is a hormone with known effects on cellular processes.
Purpose of the Study:
- To investigate the effect of hydrocortisone on hyaluronate synthesis in human dermal matrix.
- To explore the molecular mechanisms underlying hydrocortisone's impact on HA production.
Main Methods:
- Human dermal fibroblasts cultured in serum-free conditions with varying hydrocortisone concentrations.
- Characterization of resultant human dermal matrices.
- Differential gene array analysis to identify molecular targets.
Main Results:
- Low hydrocortisone concentrations enhanced hyaluronate synthesis.
- High hydrocortisone concentrations inhibited hyaluronate synthesis, affecting 43 genes.
- Chondroitin sulphate synthesis remained unaffected by hydrocortisone levels.
Conclusions:
- Hydrocortisone exhibits a dose-dependent effect on hyaluronate synthesis.
- High-dose hydrocortisone may inhibit HA synthesis by downregulating key glycolytic enzymes, impacting precursor availability.
- Further research is warranted to fully elucidate the molecular mechanisms.
