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Extraction and Analysis of Taiwanese Green Propolis
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Published on: January 7, 2019

Enterococcus saccharolyticus subsp. taiwanensis subsp. nov., isolated from broccoli.

Yi-Sheng Chen1, Yu-Hsuan Lin1, Shwu-Fen Pan1

  • 1Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Road, Gui-Shan Township, Taoyuan County 333, Taiwan.

International Journal of Systematic and Evolutionary Microbiology
|August 21, 2013
PubMed
Summary
This summary is machine-generated.

A novel subspecies of Enterococcus, Enterococcus saccharolyticus subsp. taiwanensis subsp. nov., was identified from broccoli. This finding also led to an updated description of the Enterococcus saccharolyticus species.

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Published on: December 23, 2022

Area of Science:

  • Microbiology
  • Bacteriology
  • Taxonomy

Background:

  • Initial identification of a broccoli-isolated coccal strain as Enterococcus saccharolyticus was questioned.
  • Phenotypic and molecular data did not align with the established E. saccharolyticus type strain.

Purpose of the Study:

  • To molecularly and phenotypically characterize a novel coccal strain isolated from broccoli.
  • To determine if the strain represents a new taxon within the Enterococcus genus.
  • To provide an emended description of Enterococcus saccharolyticus.

Main Methods:

  • DNA-DNA hybridization
  • DNA G+C content determination
  • Phylogenetic analysis using 16S rRNA, pheS, and rpoA gene sequences
  • rep-PCR fingerprinting
  • Analysis of cellular fatty acids, whole-cell proteins, enzyme activities, and carbohydrate metabolism.

Main Results:

  • The strain showed only 76.4% DNA-DNA relatedness to the E. saccharolyticus type strain.
  • Phylogenetic analyses and phenotypic characterizations confirmed the strain's distinctness.
  • The novel subspecies Enterococcus saccharolyticus subsp. taiwanensis subsp. nov. was proposed, with strain 812(T) as the type strain.
  • An emended description of Enterococcus saccharolyticus and the proposal of Enterococcus saccharolyticus subsp. saccharolyticus subsp. nov. were also presented.

Conclusions:

  • The isolated strain represents a novel subspecies, Enterococcus saccharolyticus subsp. taiwanensis subsp. nov.
  • The study provides a refined understanding of the Enterococcus saccharolyticus species complex.
  • This research contributes to bacterial taxonomy and the characterization of foodborne microorganisms.