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Updated: May 8, 2026

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
Published on: January 11, 2017
A luminol chemiluminescence method for sensing histidine and lysine using enzyme reactions
Akimitsu Kugimiya1, Rie Fukada, Daiki Funamoto
1Center for Industry and Public Relations, Hiroshima City University, Naka-ku, Hiroshima 730-0052, Japan.
Abstract:
The analysis of free amino acids in urine and plasma is useful for estimating disease status in clinical diagnoses. Changes in the concentration of free amino acids in foods are also useful markers of freshness, nutrition, and taste. In this study, the specific interaction between aminoacyl-tRNA synthetase (aaRS) and its corresponding amino acid was used to measure amino acid concentrations. Pyrophosphate released by the amino acid-aaRS binding reaction was detected by luminol chemiluminescence; the method provided selective quantitation of 1.0-30 μM histidine and 1.0-60 μM lysine.
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