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Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Derivation of Human Embryonic Stem Cells by Immunosurgery
11:56

Derivation of Human Embryonic Stem Cells by Immunosurgery

Published on: December 13, 2007

Goat embryonic stem-like cell derivation and characterization.

Esmail Behboodi1, Louise Lam, William G Gavin

  • 1Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|August 27, 2013
PubMed
Summary
This summary is machine-generated.

Researchers successfully derived and characterized goat embryonic stem (ES) cells, demonstrating their pluripotency. This breakthrough addresses challenges in establishing farm animal ES cell lines for potential agricultural and biomedical applications.

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Area of Science:

  • Reproductive biology
  • Stem cell research
  • Animal biotechnology

Background:

  • Embryonic stem (ES) cells are pluripotent cells derived from preimplantation embryos, capable of differentiating into all cell types.
  • Establishing ES cell lines in farm animals has been challenging, with few cell lines exhibiting true pluripotency markers.
  • Existing methods for derivation, propagation, and differentiation of farm animal ES cells are not fully established.

Purpose of the Study:

  • To describe methods for isolating and characterizing goat embryonic stem (gES) cell lines.
  • To identify markers indicative of pluripotency in gES cells.
  • To demonstrate the differentiation potential of gES cells.

Main Methods:

  • Isolation of gES cell lines from in vivo-derived blastocysts.
  • Characterization of gES cells using pluripotency markers.
  • In vivo differentiation of gES cells through teratoma formation.

Main Results:

  • Successful isolation of goat ES cell lines from blastocysts.
  • Identification of specific markers associated with pluripotency in gES cells.
  • Demonstration of gES cell differentiation into all three germ layers via teratoma formation.

Conclusions:

  • The study successfully established and characterized goat embryonic stem (gES) cell lines.
  • These gES cells exhibit key markers and functional characteristics of pluripotency.
  • The findings provide a foundation for utilizing gES cells in farm animal biotechnology and research.