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Related Experiment Video

Updated: May 8, 2026

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Published on: May 16, 2019

A chemically defined medium for rabbit embryo cryopreservation.

Pierre Bruyère¹, Anne Baudot, Thierry Joly

¹UPSP 2011.03.10 (ICE), VetAgro Sup (Université de Lyon), Marcy l'étoile, France.

|August 27, 2013

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CRYO3, a synthetic substitute, offers a stable and effective alternative to fetal calf serum (FCS) for rabbit embryo cryopreservation. This new solution demonstrated superior in vivo implantation and live-fetus rates, making it a promising advancement in reproductive technologies.

Area of Science:

Reproductive Biology
Cryobiology
Biotechnology

Background:

Cryopreservation of embryos is crucial for assisted reproductive technologies.
Current solutions often rely on animal-derived products like fetal calf serum (FCS), which exhibit significant batch-to-batch variability.
This variability can impact the efficacy and reproducibility of cryopreservation protocols.

Purpose of the Study:

To evaluate CRYO3, a novel synthetic substitute, as an alternative to animal-based products in rabbit embryo cryopreservation.
To compare the thermodynamic and biological efficacy of CRYO3-based solutions against FCS-based solutions.
To assess the stability and performance of CRYO3 in both in vitro and in vivo rabbit embryo cryopreservation.

Main Methods:

A thermodynamic approach using differential scanning calorimetry was employed.

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Last Updated: May 8, 2026

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A biological approach involved slow-freezing of rabbit embryos.

Solutions containing CRYO3 or different batches of FCS were prepared in Dulbecco's phosphate buffer saline with Dimethyl Sulfoxide and evaluated for melting temperature, crystallization enthalpy, embryo survival, implantation, and live-fetus rates.

Main Results:

CRYO3 solutions exhibited similar thermodynamic properties to FCS solutions but with greater stability and no aberrant values.
In vitro embryo survival rates were comparable between CRYO3 and FCS groups.
CRYO3 significantly improved in vivo implantation rates (21.8% vs 7.1%) and live-fetus rates (18.8% vs 5.3%) compared to FCS, with a higher pregnancy rate (81.3% vs 43.8%).

Conclusions:

CRYO3 serves as a chemically defined and stable substitute for animal-based products in rabbit embryo cryopreservation.
The synthetic substitute demonstrates superior in vivo reproductive outcomes compared to traditional FCS-based solutions.
CRYO3 represents a promising advancement for improving the efficiency and reliability of embryo cryopreservation protocols.