Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination01:51

Determination

18.1K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
18.1K
Gastrulation01:56

Gastrulation

56.5K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
56.5K
Cleavage and Blastulation01:33

Cleavage and Blastulation

44.7K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
44.7K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
Epistasis01:39

Epistasis

45.6K
In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
45.6K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defective plasticity in dermatomyositis patients muscle stem cells is associated with sustained intrinsic inflammatory signaling and disruption of the histone H3.3 chromatin loading pathway.

NAR molecular medicine·2026
Same author

Driving Naive State Induction Using Human Wharton Jelly-Mesenchymal Stem Cell-Derived Conditioned Medium in Rhesus Monkey Embryonic Stem Cells.

Cells·2026
Same author

Remodeling of <i>XIST</i> regulatory landscape during primate evolution.

Science advances·2026
Same author

Atrazine Induces Reproductive Toxicity in an <i>In Vitro</i> Spermatogenesis (IVS) Model.

Biomedicines·2025
Same author

Advanced medical treatments for hair loss.

Cell transplantation·2025
Same author

Characterization of Rhesus Macaque Embryonic Stem Cells in Primed and Naïve-like Cell States of Pluripotency Using Fourier Transform Infrared (FTIR) Microspectroscopy.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Jun 3, 2025

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

10.8K

H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts.

Wilhelm Bouchereau1, Hong-Thu Pham1, Worawalan Samruan1,2

  • 1Univ Lyon, Université Lyon 1, INSERM, Stem Cell and Brain Research Institute U1208, INRAE USC 1361, Bron, F-69500, France.

Epigenetics & Chromatin
|January 12, 2025
PubMed
Summary

Histone H3 modifications at lysine 9 regulate cell fate in mammalian blastocysts. Changes in H3K9 acetylation and methylation are crucial for primitive endoderm segregation and epiblast expansion during early development.

Keywords:
EmbryoEndodermEpiblastH3K9 post-translational modificationsHistone deacetylaseHistone methyltransferaseRabbit

More Related Videos

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

6.3K
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
10:59

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Published on: November 18, 2013

18.1K

Related Experiment Videos

Last Updated: Jun 3, 2025

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
11:25

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

10.8K
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
12:09

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Published on: August 10, 2022

6.3K
Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
10:59

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Published on: November 18, 2013

18.1K

Area of Science:

  • Epigenetics
  • Developmental Biology
  • Mammalian Embryogenesis

Background:

  • Post-translational modifications of histone H3 on lysine 9, including acetylation (H3K9ac) and tri-methylation (H3K9me3), are key regulators of chromatin accessibility.
  • The specific roles of these histone modifications in mammalian blastocyst lineage segregation are not well understood.

Purpose of the Study:

  • To investigate the dynamic changes and functional significance of histone H3 lysine 9 modifications during rabbit blastocyst development.
  • To elucidate the involvement of H3K9ac, H3K9me2, and H3K9me3 in the segregation of inner cell mass lineages.

Main Methods:

  • Quantitative analysis of H3K9ac, H3K9me2, and H3K9me3 levels during rabbit blastocyst cavitation and expansion.
  • Pharmacological inhibition and enhancement of H3K9 methylation and acetylation pathways.
  • Assessment of gene expression related to pluripotency and lineage determination.

Main Results:

  • H3K9me2 and H3K9me3 levels decrease during rabbit blastocyst expansion, with H3K9me3 notably low in inner cell mass cells before increasing prior to gastrulation.
  • H3K9ac is abundant in early blastocysts but diminishes during the inner cell mass to epiblast transition.
  • Inhibition of H3K9me2/3 disrupts primitive endoderm segregation, while enhanced H3K9ac promotes epiblast expansion over primitive endoderm formation.

Conclusions:

  • Dynamic changes in H3K9 methylation and acetylation are critical for regulating lineage segregation in the mammalian blastocyst.
  • These epigenetic modifications influence the expression of genes involved in pluripotency and cell fate determination, highlighting their importance in embryonic development.