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Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Driving Naive State Induction Using Human Wharton Jelly-Mesenchymal Stem Cell-Derived Conditioned Medium in Rhesus

Preeyanan Anwised1, Ratree Moorawong1, Worawalan Samruan1

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Area of Science:

  • Stem cell biology
  • Developmental biology
  • Regenerative medicine

Background:

  • Converting primed pluripotent stem cells to a naive-like state is crucial for regenerative medicine.
  • Conditioned media (CM) offers a supportive microenvironment for stem cell state transitions.
  • Human Wharton's Jelly-derived mesenchymal stem cell-conditioned media (hWJ-MSCs-CM) and mouse embryonic fibroblast-conditioned media (MEFs-CM) are explored for this purpose.

Purpose of the Study:

  • To investigate the efficacy of hWJ-MSCs-CM and MEFs-CM in converting primed rhesus monkey embryonic stem cells (rhESCs) to a naive-like state.
  • To assess the impact of different conditioned media on pluripotency marker expression and epigenetic reprogramming.
  • To evaluate the potential of CM-based approaches for non-manipulative stem cell state induction.

Main Methods:

  • Culturing primed rhESCs under feeder-free and feeder conditions using hWJ-MSCs-CM and MEFs-CM.
  • Morphological assessment of rhESCs during conversion.
  • Immunofluorescence analysis for pluripotency and naive markers.
  • Gene expression analysis to quantify naive and primed markers.
  • Epigenetic reprogramming assessment.

Main Results:

  • Distinct morphological changes observed in rhESCs cultured with hWJ-MSCs-CM and MEFs-CM.
  • Expression of pluripotency and naive markers confirmed via immunofluorescence.
  • Gene expression analysis showed upregulation of naive markers and downregulation of primed markers.
  • Differential epigenetic reprogramming effects observed between CM sources.
  • hWJ-MSCs-CM demonstrated potential for naive-like state induction.

Conclusions:

  • hWJ-MSCs-CM supports the conversion of primed rhESCs to a naive-like state.
  • CM-based approaches offer a promising strategy for inducing naive pluripotency without genetic modification.
  • Further research into CM components could optimize primate ESC reprogramming for regenerative medicine applications.