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Updated: May 8, 2026

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

Cell detachment: post-isolation challenges.

Qin Zheng¹, Samir M Iqbal, Yuan Wan

¹Department of Oncology, The Second Affiliated Hospital of Southeast University, Nanjing, Jiangsu 210003, PR China.

Biotechnology Advances

|August 28, 2013

Summary

This summary is machine-generated.

Researchers are improving rare cell isolation techniques using nanotechnology and microfluidics. Cell detachment from substrates is a critical step for viability and subsequent analysis in disease diagnosis and therapy.

Keywords:

Aptamers Cell collection Cellular viability Enzymolysis Microfluidic devices Rare cells Stimuli-responsive polymers

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Isolation of Valvular Endothelial Cells

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Published on: December 29, 2010

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Last Updated: May 8, 2026

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

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Published on: April 18, 2016

Isolation of Valvular Endothelial Cells

Isolation of Valvular Endothelial Cells

Published on: December 29, 2010

Area of Science:

Biotechnology
Nanotechnology
Microfluidics

Background:

Rare cells are crucial biomarkers for disease diagnosis, prognosis, and personalized therapy.
Effective detection and isolation of rare cells are critical research areas.
Advancements in nanotechnology and microfluidics offer promising solutions for rare cell analysis.

Purpose of the Study:

To review various cell detachment methods for post-isolation analysis.
To highlight the importance of cell detachment in rare cell isolation techniques.
To guide the selection of substrates and methods for effective cell sorting.

Main Methods:

Review of existing literature on cell detachment techniques.
Analysis of methods for detaching rare cells from substrates while maintaining viability.
Discussion of integrated and automated systems for comprehensive rare cell isolation and analysis.

Main Results:

Cell detachment is a critical challenge in post-isolation analysis of rare cells.
Various methods exist for detaching cells, each with implications for cell viability.
The choice of substrate and detachment method significantly impacts the success of cell sorting.

Conclusions:

Optimizing cell detachment is essential for advancing rare cell isolation and analysis.
Nanotechnology and microfluidics are key enablers for integrated rare cell sorting systems.
Further research into robust and gentle cell detachment methods is warranted for clinical applications.