Parameter conversion from Kedem-Katchalsky model to two-parameter model revisited

Area of Science:

• Biophysics
• Cell Biology
• Cryobiology

Background:

• Successful cryopreservation requires minimizing osmotic injury from cryoprotective agents.
• The Kedem-Katchalsky (KK) and two-parameter (2P) models describe cell volumetric responses.
• The 2P model is simpler and often preferred, but requires accurate parameters.

Purpose of the Study:

• To verify and correct the 2P model parameters for various cell types.
• To address inaccuracies in previous derivations of 2P parameters from KK parameters.
• To provide a reliable dataset for optimizing cryopreservation protocols.

Main Methods:

• Re-evaluation of 2P model parameter calculations based on original experimental conditions.
• Comparison and correction of previously published 2P parameter values.
• Generation of a verified dataset for multiple cell types.

Main Results:

• Identified and corrected discrepancies in 2P model parameters derived from KK parameters.
• Presented a revised dataset for cell volumetric responses crucial for cryopreservation.
• The corrected dataset enhances the predictive accuracy of the 2P model.

Conclusions:

• Accurate cell parameters are essential for reliable cryopreservation modeling.
• The corrected dataset facilitates improved optimization of cryoprotective agent addition and removal.
• This work provides a foundation for more precise cryobiology research and applications.