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A Multimodal Wide-Field Fourier-Transform Raman Microscope
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Published on: December 30, 2025

Fourier phase microscopy with white light.

Basanta Bhaduri1, Krishnarao Tangella, Gabriel Popescu

  • 1Quantitative Light Imaging Laboratory, Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Biomedical Optics Express
|September 7, 2013
PubMed
Summary
This summary is machine-generated.

We developed white light Fourier phase microscopy (wFPM) to overcome speckle noise in laser-based methods. This technique provides high-resolution phase imaging at 12.5 frames per second for dynamic biological sample analysis.

Keywords:
(070.0070) Fourier optics and signal processing(070.6120) Spatial light modulators(120.5050) Phase measurement(170.0180) Microscopy

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Area of Science:

  • Optical microscopy
  • Biomedical imaging
  • Phase contrast microscopy

Background:

  • Laser-based Fourier phase microscopy (FPM) utilizes spatial component decomposition for phase imaging.
  • Coherent illumination in FPM leads to speckle-induced contrast degradation.
  • Need for advanced microscopy techniques for dynamic, high-resolution biological sample analysis.

Purpose of the Study:

  • To introduce and characterize white light Fourier phase microscopy (wFPM) as an alternative to laser-based FPM.
  • To demonstrate wFPM's capability for high-speed, high-resolution quantitative phase imaging.
  • To showcase wFPM's utility in contrast enhancement and dynamic phase measurement of biological samples.

Main Methods:

  • Implementation of wFPM using spatially coherent white light.
  • Utilized a fast spatial light modulator (SLM) and a scientific-grade complementary metal oxide semiconductor (sCMOS) camera.
  • Achieved quantitative phase imaging at 12.5 frames per second with 5.5-megapixel resolution.

Main Results:

  • wFPM offers high spatial phase sensitivity due to low temporal coherence.
  • Common path geometry ensures high temporal phase stability, reducing speckle noise.
  • Demonstrated successful imaging of benign colonic glands and red blood cell membrane fluctuations.

Conclusions:

  • wFPM effectively mitigates speckle noise inherent in laser-based FPM.
  • The developed system enables rapid, high-resolution quantitative phase imaging of dynamic biological processes.
  • wFPM serves as a valuable tool for contrast enhancement and dynamic phase measurements in biological research.