Colony PCR

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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Megan Bergkessel1, Christine Guthrie

  • 1Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.

Methods in Enzymology
|September 10, 2013
PubMed

Abstract:

Colony PCR is a method for rapidly screening colonies of yeast or bacteria that have grown up on selective media following a transformation step, to verify that the desired genetic construct is present, or to amplify a portion of the construct.

Keywords:
Colony PCRDNA extractionDNA-intercalating dyePrimer synthesisProduct visualizationThermostable polymerase

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