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Related Concept Videos

PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
PCR01:32

PCR

Overview
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: May 8, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Colony PCR.

Megan Bergkessel1, Christine Guthrie

  • 1Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.

Methods in Enzymology
|September 10, 2013
PubMed
Summary
This summary is machine-generated.

Colony PCR quickly screens yeast and bacterial colonies after transformation. This molecular biology technique confirms the presence of genetic constructs or amplifies specific DNA portions.

Keywords:
Colony PCRDNA extractionDNA-intercalating dyePrimer synthesisProduct visualizationThermostable polymerase

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Last Updated: May 8, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells

Published on: March 3, 2015

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Background:

  • Transformation is a key step in genetic engineering.
  • Screening is necessary to identify successful transformants.
  • Traditional screening methods can be time-consuming.

Purpose of the Study:

  • To describe Colony PCR as a rapid screening method.
  • To highlight its utility in verifying genetic constructs.
  • To explain its application in amplifying construct portions.

Main Methods:

  • Colony PCR involves direct amplification from microbial colonies.
  • Utilizes polymerase chain reaction (PCR) on bacterial or yeast colonies.
  • Requires minimal sample preparation.

Main Results:

  • Rapid confirmation of desired genetic constructs.
  • Efficient amplification of specific DNA fragments.
  • Reduces time and resources compared to other methods.

Conclusions:

  • Colony PCR is an effective and efficient screening tool.
  • It streamlines the process of genetic manipulation in yeast and bacteria.
  • Facilitates faster research and development in molecular biology.