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Published on: January 20, 2019
[Immunogenicity of porcine fibrinogen]
Feifei Wang1, Hongqun Qiao, Xuejun He
1College of Pharmaceutical Sciences, Nanjing University of Technology, Nanjing, China.
Objective:
To investigate the immunogenicity and immune effect of porcine fibrinogen and provide experimental evidence for safety evaluation and further optimization of the drug.
Methods:
An improved ABC-ELISA system of biotin and conjugated streptomyces avidin was used for detecting antibodies. The serum antibody titer and its dynamics were studied by means of the injury experiment in New Zealand rabbits. Effects of porcine fibrinogen on immune system of mice were evaluated by analyzing the weight gain and indexes of immune organs, the proliferation of spleen lymphocytes by thiazole blue (MTT) chromatometry and the neutrophil phagocytosis by nitro-tetrazolium chloride blue (NTB) reduction method.
Results:
The antibodies could be detected in New Zealand white rabbit serum 1 week after immunization, and antibody levels decreased to a more stable level after it peaked at the second week, at the same time, the antibody titer also showed the same trend. The weight and indexes of immune organs were not changed significantly in mice. Compared with the control group, the proliferation rate of lymphocytes in each experimental group didn't show statistical difference, but neutrophil phagocytosis of the experimental groups was enhanced significantly.
Conclusion:
The humoral immune response was induced in New Zealand rabbits after stimulated with porcine fibrinogen, but no specific immune response was found in mice.
