Related Experiment Video

Updated: May 8, 2026

Human Neutrophil Flow Chamber Adhesion Assay
12:42

Human Neutrophil Flow Chamber Adhesion Assay

Published on: July 2, 2014

[Neutrophils expression of adhesion molecules in diabetic nephropaty patients]

T D Shcherban'

    Georgian Medical News
    |September 10, 2013
    PubMed

    Insights

    Diabetic nephropathy (DN) increases CD11b and CD54 positive neutrophils, impacting cellular cooperation. This highlights how hyperglycemia-driven adhesion molecule expression contributes to vascular injury in DN.

    Area of Science:

    • Immunology
    • Nephrology
    • Endocrinology

    Background:

    • Diabetic nephropathy (DN) is a major complication of diabetes mellitus.
    • Neutrophils play a role in inflammation and tissue damage.
    • Adhesion molecules on neutrophils are implicated in vascular injury.

    Purpose of the Study:

    • To investigate the expression of CD11b and CD54 on neutrophils in patients with DN.
    • To explore the relationship between hyperglycemia, adhesion molecule expression, and vascular injury in DN.
    • To understand the role of cellular cooperation in the pathogenesis of DN.

    Main Methods:

    • Flow cytometry was used to quantify CD11b and CD54 expression on neutrophils.
    • Patients with DN, arterial hypertension, and healthy donors were included in the study.
    • Morphological and biochemical markers of intercellular cooperation were assessed.

    Main Results:

    • Neutrophils from DN patients showed increased CD11b and CD54 expression compared to controls.
    • Diabetic microenvironment conditions led to a substantial rise in adhesion molecule expression.
    • Evidence of impaired cellular cooperation was observed in DN patients.

    Conclusions:

    • Increased CD11b and CD54 expression on neutrophils is associated with DN development.
    • Hyperglycemia-induced adhesion molecule upregulation may mediate vascular and tissue injury in DN.
    • Identifying markers of intercellular cooperation can deepen the understanding of DN pathogenesis.

    More Related Videos

    Quantification of Diabetes-induced Adherent Leukocytes in Retinal Vasculature05:54

    Quantification of Diabetes-induced Adherent Leukocytes in Retinal Vasculature

    Published on: January 24, 2025

    Related Concept Videos

    Diabetic Nephropathy01:28

    Diabetic Nephropathy

    Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration occur due to afferent arteriolar...
    Diabetic Neuropathy01:22

    Diabetic Neuropathy

    DefinitionDiabetic neuropathy is nerve damage caused by long-standing diabetes mellitus. It results directly from prolonged high blood sugar levels.PathophysiologyThe pathophysiology of diabetic neuropathy involves both metabolic and vascular disturbances triggered by chronic hyperglycemia.Metabolic injury: Elevated glucose levels activate the polyol pathway within nerve cells, leading to the accumulation of sorbitol and fructose. This increases oxidative stress, disrupts normal nerve...
    Selectins01:25

    Selectins

    Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
    Diabetic Retinopathy01:27

    Diabetic Retinopathy

    DefinitionDiabetic retinopathy is a microvascular complication of diabetes affecting the retinal blood vessels.Risk FactorsDiabetic retinopathy is present in almost all individuals with type 1 diabetes and more than 60% of those with type 2 diabetes after two decades of disease.The risk increases with poor glycemic control, hypertension, dyslipidemia, smoking, pregnancy, and puberty.Although cataracts and glaucoma are also more frequent in people with diabetes, retinopathy remains the leading...
    Nephrotic Syndrome I : Introduction01:24

    Nephrotic Syndrome I : Introduction

    Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of fluid...
    Type I Diabetes II: Pathophysiology01:26

    Type I Diabetes II: Pathophysiology

    Type 1 diabetes mellitus arises from an immune-mediated destruction of pancreatic β-cells, resulting in an absolute deficiency of insulin. This process develops in genetically susceptible individuals when autoimmunity, environmental exposures, and immunologic dysregulation converge to trigger a targeted attack on the insulin-producing cells of the pancreas. The β-cells are located within the islets of Langerhans and are essential for regulating blood glucose by facilitating cellular uptake of...