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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Post-column labeling techniques in amino acid analysis by liquid chromatography
1Biotoxins and Analytical Chemistry Laboratory, Department of Fisheries and Aquaculture Technology, Alexander Technological Educational Institute of Thessaloniki, 1 N. Miltiadi St., 63200, N. Moudania, Greece, rigas@aqua.teithe.gr.
Analytical and Bioanalytical Chemistry
|September 10, 2013
Summary
Post-column labeling techniques in liquid chromatography offer a robust and sensitive method for amino acid analysis (AAA). This approach enhances separation and detection, overcoming challenges in analyzing diverse amino acid structures.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Amino acid analysis (AAA) presents significant challenges in sample preparation, separation, and detection due to the diverse chemical properties of amino acids.
- Liquid chromatography (LC) is a prevalent technique for AAA, utilizing pre-column or post-column labeling to enhance separation, selectivity, and sensitivity.
Purpose of the Study:
- To review current separation methods for AAA, focusing on post-column labeling techniques coupled with LC.
- To compare post-column labeling with pre-column methods and discuss strategies for developing effective post-column derivatization (PCD).
Main Methods:
- Focus on LC methods combined with post-column labeling techniques for AAA.
- Investigation of reaction conditions for optimal PCD to improve sensitivity and selectivity.
- Utilization of various detectors, including UV-Vis, fluorescence, and electrochemical detectors.
Main Results:
- Post-column labeling techniques provide more rugged and reproducible AAA compared to pre-column methods.
- Optimized PCD conditions significantly enhance sensitivity and selectivity in amino acid separation.
- Demonstration of the versatility of PCD methods for practical amino acid analysis across different detectors.
Conclusions:
- LC coupled with post-column labeling is a highly effective strategy for overcoming AAA challenges.
- PCD offers superior performance in terms of robustness, reproducibility, sensitivity, and selectivity for amino acid analysis.
- The review highlights the practical applicability and versatility of PCD methods in modern analytical chemistry.
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