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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Fluorescence enhancement using silver-gold nanocomposite substrates.

Sharmistha Dutta Choudhury1, Ramachandram Badugu, Krishanu Ray

  • 1Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA.

Proceedings of Spie--The International Society for Optical Engineering
|September 13, 2013
PubMed
Summary

Metal-enhanced fluorescence (MEF) using silver-gold nanocomposites (Ag-Au-NCs) significantly boosts dye brightness. The level of residual silver in Ag-Au-NCs controls the observed fluorescence enhancement for MEF applications.

Keywords:
Silver-gold nanocompositesgalvanic replacementmetal enhanced fluorescenceplasmon controlled fluorescenceplasmonics

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Area of Science:

  • Plasmonics
  • Nanotechnology
  • Spectroscopy

Background:

  • Metal-enhanced fluorescence (MEF) leverages plasmonic nanostructures to amplify fluorophore signals.
  • Developing efficient plasmonic substrates is crucial for advancing MEF applications.

Purpose of the Study:

  • To investigate silver-gold nanocomposite (Ag-Au-NC) structures as plasmonic substrates for MEF.
  • To explore the effect of residual silver on MEF performance.

Main Methods:

  • Fabrication of Ag-Au-NC substrates via galvanic replacement reaction of silver with gold.
  • Characterization of MEF using ATTO655 and Cy5 dyes.
  • Analysis of fluorescence intensity and lifetime changes.

Main Results:

  • Observed significant enhancement in fluorescence intensity for ATTO655 and Cy5.
  • Reported a decrease in fluorescence lifetimes of the dyes.
  • Demonstrated that residual silver content in Ag-Au-NCs influences the degree of fluorescence enhancement.

Conclusions:

  • Galvanic replacement reaction provides a facile route to synthesize effective Ag-Au-NC substrates for MEF.
  • Ag-Au-NC substrates show promise for various MEF-based applications.
  • Controlling residual silver is key to optimizing MEF performance.