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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Plasmon-controlled fluorescence and single DNA strand sequenching.

Nuriye Akbay¹, Krishanu Ray, Mustafa H Chowdhury

¹Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, MD 21201, USA.

Proceedings of Spie--The International Society for Optical Engineering

|September 13, 2013

Summary

This summary is machine-generated.

Metallic nanoparticles significantly boost UV fluorophore emission, enhancing brightness and photostability. This metal-enhanced fluorescence (MEF) effect was observed for UV fluorophores and DNA bases, showing promise for advanced optical applications.

Keywords:

DNA sequenching fluorescence metal enhanced fluorescence nanotechnology plasmon controlled fluorescence plasmonics

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Area of Science:

Plasmonics and Nanophotonics
Biophysics and Spectroscopy
Materials Science

Background:

Metal-enhanced fluorescence (MEF) has been demonstrated using visible and near-infrared (NIR) fluorophores.
Metallic nanostructures and nanoparticles alter fluorophore photophysical properties, increasing brightness and photostability.
Previous studies focused on visible and NIR wavelengths, leaving UV applications less explored.

Purpose of the Study:

To extend MEF studies to ultraviolet (UV) wavelengths.
To investigate the enhancement of UV fluorophores, including intrinsic protein fluorescence, using aluminum and platinum nanoparticles.
To examine the effect of metallic nanoparticles on the fluorescence intensity of DNA bases and DNA G-quadruplex structures.

Main Methods:

Ensemble spectroscopic studies to measure fluorescence.
Finite-difference time-domain (FDTD) simulations to model nanoparticle effects on UV fluorophores.
Steady-state fluorescence emission measurements on DNA bases and G-quadruplexes on metallic substrates.

Main Results:

Aluminum and platinum particles were shown to enhance UV fluorophore emission (300-420 nm), including intrinsic protein fluorescence.
FDTD calculations confirmed the plasmonic effects of aluminum nanoparticles on nearby UV emitters.
Fluorescence intensities of DNA bases increased 2- to 3-fold, and DNA G-quadruplex fluorescence increased approximately 5-fold on Al and Pt substrates compared to quartz controls.

Conclusions:

Metallic nanoparticles, specifically aluminum and platinum, effectively enhance fluorescence in the UV spectrum.
MEF principles are applicable to UV fluorophores and biomolecules like DNA bases and G-quadruplexes.
This work expands the utility of MEF for UV applications and offers new possibilities for biosensing and imaging.