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Updated: May 7, 2026

08:09
A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins
Published on: January 7, 2019
Detection of salt bridges to lysines in solution in barnase
Mike P Williamson1, Andrea M Hounslow, Joe Ford
1Dept of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK. m.williamson@sheffield.ac.uk.
Abstract:
We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.

