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Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
Published on: July 20, 2016
Selective functionalization of the protein N terminus with N-myristoyl transferase for bioconjugation in cell lysate
Chethana Kulkarni1, Tamara L Kinzer-Ursem, David A Tirrell
1Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125 (USA) http://tirrell-lab.caltech.edu.
Chembiochem : a European Journal of Chemical Biology
|September 14, 2013
Abstract:
A site to behold: Robust site-specific functionalization of engineered proteins is achieved with N-myristoyl transferase (NMT) in bacterial cells. NMT tolerates non-natural substrate proteins as well as reactive fatty acid tags, rendering it a powerful tool for protein conjugation applications, including the construction of protein microarrays from lysate.
