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Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Published on: April 16, 2012
Restricted expression of p55 interleukin 2 receptor (CD25) on normal T cells
A L Jackson1, H Matsumoto, M Janszen
1Becton Dickinson Monoclonal Center, Incorporated, Mountain View, California 94043.
Clinical Immunology and Immunopathology
|January 1, 1990
Summary
Normal lymphocytes show 30% positivity for the interleukin 2 receptor p55 chain. Most of these cells are CD4+ T cells, primarily within the CD45RA-negative subset, indicating an activated or memory T cell phenotype.
Area of Science:
- Immunology
- Cell Biology
Background:
- The interleukin 2 receptor (IL-2R) plays a crucial role in T cell activation and proliferation.
- The p55 chain (CD25) is a component of the high-affinity IL-2R, often used as a marker for activated T cells.
Purpose of the Study:
- To quantify the expression of the interleukin 2 receptor p55 chain on normal blood lymphocytes.
- To characterize the T cell subsets expressing this marker.
Main Methods:
- Flow cytometry was employed using a phycoerythrin-conjugated antibody specific for the p55 chain of the IL-2 receptor.
- Immunophenotyping was performed to identify CD4 and CD45RA expression on positive cells.
Main Results:
- An average of 30% of normal blood lymphocytes were positive for the IL-2 receptor p55 chain.
- Of the IL-2R p55 chain-positive cells, two-thirds were identified within the CD4 T cell subset.
- The majority of these CD4+ cells were found in the CD45RA-negative fraction.
Conclusions:
- A significant proportion of normal circulating lymphocytes express the IL-2 receptor p55 chain.
- This expression is predominantly found on CD4+ T cells that exhibit characteristics of activation or memory phenotype (CD45RA negative).
- These findings contribute to understanding T cell populations in healthy individuals.
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