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Sandwich assay for mixed-sequence recognition of double-stranded DNA: invader-based detection of targets specific to

Benjamin Denn1, Saswata Karmakar, Dale C Guenther

  • 1Department of Chemistry, University of Idaho, 875 Perimeter Drive MS 2343, Moscow, ID 83844-2343, USA. hrdlicka@uidaho.edu.

Chemical Communications (Cambridge, England)
|September 17, 2013
PubMed

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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...