Related Experiment Video
Updated: Feb 13, 2026
14:57
DNA Stable-Isotope Probing DNA-SIP
Published on: August 2, 2010
45.2K
DNA-targeting Invader probes: discovery, principles and applications
Patrick J Hrdlicka1, Michaela E Everly1
1Department of Chemistry, University of Idaho Moscow Idaho 83844-2343 USA hrdlicka@uidaho.edu.
RSC Chemical Biology
|February 12, 2026
Summary
Chemically modified Invader probes bind double-stranded DNA (dsDNA) by displacing Watson-Crick base pairs. These novel probes offer potential for diagnostics and therapeutics targeting genetic diseases.
Area of Science:
- Molecular Biology
- Chemical Biology
- Genetics
Background:
- Development of sequence-unrestricted DNA-binding ligands is crucial for diagnostics and therapeutics.
- Existing methods often target DNA grooves, limiting accessibility.
- Strand-invading probes offer an alternative by displacing base pairs.
Purpose of the Study:
- To detail the discovery, principles, and applications of Invader probes.
- To present a novel class of double-stranded DNA (dsDNA)-targeting strand-invading probes.
- To highlight the potential of Invader probes in diagnostics and gene expression modulation.
Main Methods:
- Design of double-stranded oligonucleotide probes with intercalator-functionalized nucleotides.
- Engineering probes to destabilize the probe duplex while maintaining high single-strand affinity.
- Characterization of Invader probe binding to dsDNA by displacing Watson-Crick base pairs.
Main Results:
- Invader probes are designed to bind specific dsDNA regions by forming new, stable base pairs.
- The probes destabilize their own duplex structure, facilitating strand invasion.
- Individual strands of Invader probes exhibit high affinity for complementary DNA sequences.
Conclusions:
- Invader probes represent a significant advancement in dsDNA-targeting ligands.
- Their unique mechanism offers potential for novel diagnostic and therapeutic applications.
- Further research into Invader probes could lead to new treatments for genetic diseases.
More Related Videos
13:19Microarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer
Published on: November 2, 2013
17.2K
11:15In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
11.5K
Related Concept Videos
01:17Bernoulli's Principle: Applications
6.8K
There are many devices and situations in which fluid flows at a constant height and so can be analyzed using Bernoulli's principle. These devices include, but are not limited to, entrainment devices and fluid flow measuring devices.
Entrainment devices use a high fluid speed to create low pressures and, thus, entrain one fluid into another. Some examples of these devices are given below:
Entrainment devices use a high fluid speed to create low pressures and, thus, entrain one fluid into another. Some examples of these devices are given below:
6.8K
03:31Labeling DNA Probes
9.5K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.5K
01:11Design Example: Application of Archimedes' Principle
882
Archimedes' principle is fundamental in analyzing the buoyant force and stability of floating bodies. In this example, a wooden block with a rectangular section floats in seawater. Based on the block's dimensions, its specific gravity and the specific weight of seawater are used to find the volume of water displaced and the center of buoyancy.
The volume of seawater displaced by the block is determined by first calculating the block's weight. This is done by multiplying the...
The volume of seawater displaced by the block is determined by first calculating the block's weight. This is done by multiplying the...
882
04:08The Uncertainty Principle
33.1K
Werner Heisenberg considered the limits of how accurately one can measure properties of an electron or other microscopic particles. He determined that there is a fundamental limit to how accurately one can measure both a particle’s position and its momentum simultaneously. The more accurate the measurement of the momentum of a particle is known, the less accurate the position at that time is known and vice versa. This is what is now called the Heisenberg uncertainty principle. He...
33.1K
01:49Hardy-Weinberg Principle
76.6K
Diploid organisms have two alleles of each gene, one from each parent, in their somatic cells. Therefore, each individual contributes two alleles to the gene pool of the population. The gene pool of a population is the sum of every allele of all genes within that population and has some degree of variation. Genetic variation is typically expressed as a relative frequency, which is the percentage of the total population that has a given allele, genotype or phenotype.
76.6K
03:06The Pauli Exclusion Principle
59.6K
The arrangement of electrons in the orbitals of an atom is called its electron configuration. We describe an electron configuration with a symbol that contains three pieces of information:
59.6K