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Updated: May 7, 2026

10:15
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
Multiplex ligation-dependent probe amplification (MLPA) for ultrasensitive multiplexed microRNA detection using
Pengbo Zhang1, Ye Liu, Yajing Zhang
1Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P. R. China. liuch@hbu.edu.cn lzpbd@hbu.edu.cn.
Abstract:
Using the ribonucleotide-modified DNA probes, multiple microRNAs can be simultaneously detected in one ligation-based PCR reaction. As low as 0.2 fM microRNA can be accurately detected with high specificity.

