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Updated: May 7, 2026
Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
Published on: March 17, 2016
[Establishment and clinical application of TapMan real-time RT-PCR method for detection of HHV-6]
Qian-Qian Chen1, Bing Zhang, Zhi-Ping Xie
1University of South China, Hengyang 421000, China.
Objective:
To establish a rapid, sensitive and specific real-time PCR method for detection of Human Herpesvirus-6 (HHV-6).
Methods:
According to the reference, a pair of primers and a probe were designed located in U65-66 gene and to set up the standards. We established a real-time RT-PCR method for detection of HHV-6, and to verify the specificity, sensitivity, reproducibility.
Results:
The correlation coefficient was 0.999, E = 97.9%, the coefficient of variation values of Ct were 0.61% and 3.13% in real-time PCR assay for inter and intra assay, respectively. The results of all viruses were negative except of HHV-6 for the assay. The quantitative detection limit of the assay was 3 x 10(0) copies/microl.
Conclusion:
The real-time PCR assay is highly specific, sensitive and reproducible, which can be used to quatitative detecting clinical samples.
