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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Related Experiment Video

Updated: May 7, 2026

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Dual modality endomicroscope with optical zoom capability.

Dimitre G Ouzounov1, David R Rivera, Wendy O Williams

  • 1Schol of Applied and Engineering Physics, Cornell University, Ithaca, NY, 14853, USA.

Biomedical Optics Express
|September 20, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a miniature endomicroscope with dual imaging modes for enhanced tissue visualization. It enables navigation using a wide field-of-view and detailed cellular analysis with high-resolution imaging.

Keywords:
(110.2350) Fiber optics imaging(170.2150) Endoscopic imaging(180.4315) Nonlinear microscopy

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Video-rate Scanning Confocal Microscopy and Microendoscopy
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Area of Science:

  • Biomedical Engineering
  • Optical Imaging
  • Microscopy

Background:

  • Accurate tissue visualization is crucial for disease diagnosis.
  • Current imaging techniques may lack the necessary resolution or field-of-view for comprehensive analysis.

Purpose of the Study:

  • To develop and evaluate a novel miniature endomicroscope.
  • To combine large field-of-view (FOV) and high-resolution imaging modalities.
  • To enable efficient navigation and detailed cellular analysis of biological tissues.

Main Methods:

  • Development of a miniature endomicroscope integrating reflectance and multiphoton fluorescence imaging.
  • Utilized a large FOV mode (1.15 mm) for navigation.
  • Employed high-resolution (~0.5 μm) imaging for cellular detail resolution.
  • Acquired in vivo and ex vivo images of unstained normal and tumor tissues.

Main Results:

  • The endomicroscope successfully acquired images of unstained normal and tumor tissues.
  • The dual-mode system allowed for effective navigation and subsequent high-resolution cellular imaging.
  • Demonstrated the capability to resolve cellular details in both in vivo and ex vivo settings.

Conclusions:

  • The developed miniature endomicroscope offers a versatile tool for tissue imaging.
  • The combination of large FOV and high-resolution imaging enhances diagnostic potential.
  • This technology can aid in the study and diagnosis of various tissue pathologies.