Tri-modal microscopy with multiphoton and optical coherence microscopy/tomography for multi-scale and multi-contrast...

Area of Science:

• Biomedical Optics
• Microscopy
• Biophotonics

Background:

• Multi-scale multimodal microscopy offers versatile imaging by combining various contrasts, fields of view, and resolutions.
• Subsurface visualization of biological tissues is crucial for understanding cellular structures and tissue architecture.

Purpose of the Study:

• To present a novel tri-modal microscope integrating multiphoton microscopy (MPM), optical coherence microscopy (OCM), and optical coherence tomography (OCT).
• To demonstrate the capability of this system for subsurface visualization of biological tissues with multiple intrinsic contrasts.

Main Methods:

• The microscope combines MPM, OCM, and OCT techniques.
• It utilizes intrinsic contrasts such as scattering, two-photon excitation fluorescence (TPEF), and second harmonic generation (SHG).
• The system allows for both rapid scanning over large areas and high-resolution zoom-in on cellular structures.

Main Results:

• The tri-modal system successfully visualized multiple intrinsic contrasts in various biological samples.
• It demonstrated efficient large-area scanning and high-resolution cellular imaging.
• Label-free imaging was achieved, providing a comprehensive set of parameters for sample analysis.

Conclusions:

• The developed tri-modal microscope is a powerful tool for subsurface biological tissue visualization.
• It enables label-free imaging with multiple contrasts, facilitating reliable sample analysis.
• This technique holds significant potential for advancing research in tissue diagnostics and biological studies.