Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A technique for reembedding celloidin sections for electron microscopy.

D Portmann1, J Fayad, P A Wackym

  • 1ORL Service Centre Hospitalo-Universitaire Pellegrin, Bordeaux, France.

The Laryngoscope
|February 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A short history of nasal polyposis.

European annals of otorhinolaryngology, head and neck diseases·2023
Same author

Anatomical basis of drug delivery to the inner ear.

Hearing research·2018
Same author

Cholesteatoma and osteoradionecrosis after radio­therapy of the temporal bone: Surgical aspects.

Revue de laryngologie - otologie - rhinologie·2018
Same author

Role of BDNF and neurotrophic receptors in human inner ear development.

Cell and tissue research·2017
Same author

Experimental investigation of threaded cup performance during insertion in total hip replacement.

Hip international : the journal of clinical and experimental research on hip pathology and therapy·2017
Same author

Cholesteatoma presenting as a late complication of cochlear implant surgery: Case report and literature review.

Revue de laryngologie - otologie - rhinologie·2016
Same journal

Discrimination of Pairs of Chemosensory Stimuli in Relation to Respiration.

The Laryngoscope·2026
Same journal

What Safety Precautions Are Recommended When Lasering in the Airway?

The Laryngoscope·2026
Same journal

Success of Anterior Ethmoidal Artery Flaps for Nasal Septal Perforation Repair: A Systematic Review.

The Laryngoscope·2026
Same journal

Laryngeal IgG4-Related Disease: A Systematic Review of Clinical Features and Management.

The Laryngoscope·2026
Same journal

Elevated BMI Is Not Associated With Adverse Outcomes in Open Airway Reconstruction.

The Laryngoscope·2026
Same journal

What is the Most Effective Treatment Approach for Vocal Fold Granuloma?

The Laryngoscope·2026
See all related articles

A new method reembeds celloidin sections of human temporal bones for transmission electron microscopy (TEM). This technique enables analysis of the inner ear without dissection, though it slightly impacts ultrastructural preservation.

Area of Science:

  • Histology
  • Microscopy
  • Otolaryngology

Background:

  • Celloidin embedding is a traditional method for preserving delicate tissues like human temporal bones.
  • Transmission electron microscopy (TEM) requires high-resolution ultrastructural preservation.
  • Analyzing the intact human inner ear labyrinth presents significant technical challenges.

Purpose of the Study:

  • To describe a novel technique for reembedding celloidin-embedded human temporal bone sections for TEM analysis.
  • To evaluate the impact of this reembedding technique on ultrastructural preservation.
  • To assess the utility of the technique for analyzing intact human inner ear structures.

Main Methods:

  • A four-step reembedding process: loosening sections, removing celloidin with clove oil, staining with osmium tetroxide and tannic acid, and embedding in epoxy resin.

Related Experiment Videos

  • Transmission electron microscopy (TEM) was used to examine reembedded sections.
  • Comparison of ultrastructural preservation between optimally fixed and poorly fixed tissue.

    • Main Results:

    • The reembedding technique allows for TEM analysis of the intact human labyrinth without dissecting fragile components.
    • Autolytic changes were observed in poorly fixed specimens.
    • Optimally fixed tissues showed some degree of altered ultrastructural preservation, including less preserved cell membranes and increased cytosol electron density, slightly reducing EM resolution.

    Conclusions:

    • The described technique offers a method for TEM analysis of celloidin-embedded human temporal bones, preserving the intact labyrinth.
    • While the technique introduces minor alterations to ultrastructure, it may be valuable for histopathological analysis of freshly fixed human inner ear tissue.
    • This method could facilitate detailed ultrastructural studies of the human inner ear without compromising the integrity of the specimen.