A new, rapid and sensitive bioluminescence assay for drug screening on Leishmania
Lucie Paloque1, Nicolas Vidal, Magali Casanova
1Infections Parasitaires, Transmission, Physiopathologie et Thérapeutique, UMR MD3, Faculté de Pharmacie, Aix-Marseille Université, 27 Boulevard Jean Moulin, CS 30064, 13385 Marseille Cedex 05, France.
Journal of Microbiological Methods
|September 24, 2013
Abstract:
We validated a new method, based on luciferine/luciferase bioluminescence, for drug screening on promastigotes of different Leishmania species. Results obtained with this new, rapid, reproducible, and reliable method are in good accordance with results obtained by the conventional MTT assay. This bioluminescence assay has a lower detection limit.
