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Updated: May 7, 2026

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
07:32

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Published on: June 15, 2012

Size-selective collection of circulating tumor cells using Vortex technology.

Elodie Sollier1, Derek E Go, James Che

  • 1Department of Bioengineering, University of California, 420 Westwood Plaza, 5121 Engineering V, P.O. Box 951600, Los Angeles, CA 90095, USA. dicarlo@seas.ucla.edu.

Lab on a Chip
|September 25, 2013
PubMed
Summary

A novel Vortex-based liquid biopsy method efficiently isolates circulating tumor cells (CTCs) from blood. This low-cost, rapid technique offers high purity for cancer research and personalized treatment strategies.

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Last Updated: May 7, 2026

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Published on: May 14, 2019

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

Area of Science:

  • Biomedical Engineering
  • Oncology
  • Biotechnology

Background:

  • Current cancer imaging methods like CT and PET are radiation-intensive and costly.
  • Liquid biopsies offer a less invasive alternative for monitoring cancer progression and treatment.
  • Circulating tumor cells (CTCs) are valuable biomarkers for cancer prognosis and personalized therapy.

Purpose of the Study:

  • To develop and optimize a microfluidic device for high-purity isolation of CTCs.
  • To evaluate the efficiency and viability of CTC capture using a novel Vortex technology.
  • To demonstrate the clinical applicability of the Vortex method for enumerating CTCs in cancer patients.

Main Methods:

  • Adaptation of micro-scale vortices and inertial focusing for CTC extraction.
  • Systematic optimization of device parameters (channel dimensions, flow rates).
  • Validation of the device using cancer cell lines in blood, assessing factors like dilution and cell deformability.

Main Results:

  • Successful high-purity extraction of CTCs from breast and lung cancer patients.
  • Demonstrated cell viability and independence from EpCAM expression.
  • Achieved rapid processing (20 min/7.5 mL) with high purity (57-94%) and cell concentration.

Conclusions:

  • The Vortex approach provides a rapid, cost-effective, and high-purity method for CTC isolation.
  • This technology facilitates CTC enumeration and analysis for cancer research and clinical applications.
  • The simplicity and efficiency of the Vortex method support widespread adoption for personalized cancer care.