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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Apr 28, 2026

Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

Published on: February 11, 2015

Live intracellular super-resolution imaging using site-specific stains.

Lina Carlini¹, Suliana Manley

¹Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne , Lausanne, Switzerland.

ACS Chemical Biology

|October 2, 2013

Summary

This summary is machine-generated.

New site-specific dyes and imaging media enable dense, high-performance live-cell super-resolution (SR) imaging. These combinations effectively highlight intracellular structures for detailed visualization.

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Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Super-resolution (SR) imaging relies on photoswitchable dyes for precise molecular localization.
Effective SR requires dyes that densely label biological structures for complete imaging.

Purpose of the Study:

To evaluate site-specific dyes and imaging media for live-cell super-resolution microscopy.
To identify optimal dye-medium combinations for dense intracellular structure highlighting.

Main Methods:

Testing site-specific dyes in various live-cell compatible imaging media.
In situ evaluation of dye performance and photophysical properties for SR imaging.

Main Results:

Identification of novel dyes and imaging medium-dye combinations for live staining.
Demonstrated dense highlighting of intracellular structures with excellent photophysical performance.
Successful application in live-cell super-resolution imaging.

Conclusions:

New dye and media combinations significantly advance live-cell SR imaging capabilities.
These findings provide tools for detailed visualization of intracellular structures.
Optimized photophysical performance enables enhanced SR image quality.