Surface molecularly imprinted polymers-based electrochemical sensor for bovine hemoglobin recognition

Area of Science:

• Electrochemistry
• Biosensors
• Materials Science

Background:

• Bovine hemoglobin (BHb) detection is crucial in various applications.
• Developing selective and sensitive sensors for protein analysis remains a challenge.

Purpose of the Study:

• To develop a novel electrochemical sensor for the recognition and detection of bovine hemoglobin (BHb).
• To utilize a surface molecularly imprinted technique for enhanced sensor performance.

Main Methods:

• Fabrication of an electrochemical sensor using aldehyde group-functionalized silica microspheres on an Au electrode.
• Covalent binding of BHb as a template molecule via imine bonds.
• Deposition of polypyrrole via electropolymerization, followed by silica etching and BHb extraction to create a 3D macroporous structure.
• Characterization using Differential Pulse Voltammetry (DPV) and Electrochemical Impedance Spectroscopy (EIS) with [Fe(CN)6](3-)/[Fe(CN)6](4-) as a probe.

Main Results:

• The sensor fabrication process was successfully characterized.
• The resulting sensor exhibited fast binding kinetics due to surface-localized imprinted cavities.
• The sensor demonstrated excellent recognition capacity for BHb compared to other proteins.
• A concentration-dependent relationship was observed between BHb levels and the electrochemical signal.

Conclusions:

• A novel, highly specific electrochemical sensor for BHb detection was successfully developed.
• The molecularly imprinted polymer-based sensor offers a promising platform for sensitive and rapid protein analysis.