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Controlled Odor Mimic Permeation Systems for Olfactory Training and Field Testing
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Odorant binding proteins: a biotechnological tool for odour control.

Carla Silva1, Teresa Matamá, Nuno G Azoia

  • 1IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.

Applied Microbiology and Biotechnology
|October 5, 2013
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Summary

Porcine odorant binding protein (OBP) effectively controls textile odors and delays fragrance release. This protein successfully masked cigarette smoke smell and retained fragrances on fabrics, offering new applications for OBP technology.

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Area of Science:

  • Biochemistry
  • Materials Science
  • Textile Chemistry

Background:

  • Odorant binding proteins (OBPs) are known for their role in olfaction.
  • Their potential application in odor control and fragrance delivery systems remains largely unexplored.

Purpose of the Study:

  • To investigate the use of porcine OBP-1 for odor control and controlled fragrance release from textile surfaces.
  • To characterize the binding affinities of porcine OBP-1 to various fragrance molecules.

Main Methods:

  • Cloning and expression of the Pig OBP-1 gene in Escherichia coli.
  • Biochemical characterization and IC50 determination for fragrance binding.
  • Coating cationized cotton with porcine OBP and testing for fragrance retention and odor masking.
  • Sensory evaluation by 30 assessors for odor attenuation efficacy.

Main Results:

  • Porcine OBP-1 demonstrated strong binding to citronellyl valerate, citronellol, and benzyl benzoate.
  • Immobilized OBP on cotton delayed the release of citronellol compared to controls.
  • Over 60% of assessors perceived a significant reduction in cigarette smoke odor on OBP-treated fabrics.

Conclusions:

  • Porcine OBP is a viable solution for mitigating unpleasant textile odors and can serve as a controlled release system.
  • OBPs represent a novel class of proteins with potential for advanced textile applications in odor management and fragrance delivery.