Imaging of noncovalent complexes by MALDI-MS.
Shelley N Jackson1, Amina S Woods
1National Institute on Drug Abuse, Intramural Research Program, National Institutes of Health, Baltimore, MD, 21224, USA.
Journal of the American Society for Mass Spectrometry
|October 5, 2013
Summary
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) was used to map noncovalent complexes (NCXs) in peptide and protein samples. This technique visualizes the spatial distribution and formation of these molecular assemblies within a sample spot.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Molecular Biology
Background:
- Noncovalent interactions are fundamental to molecular communication and biological processes.
- Mass spectrometry (MS) is a powerful technique for analyzing noncovalent complexes (NCXs).
- Electrospray ionization mass spectrometry (ESI-MS) is commonly used for NCX analysis due to its soft ionization capabilities.
Purpose of the Study:
- To investigate the spatial localization and formation of noncovalent complexes (NCXs) within a MALDI sample spot.
- To explore the utility of MALDI imaging mass spectrometry (MALDI-IMS) for studying peptide and protein NCXs.
- To understand the influence of experimental factors on NCX analysis using MALDI-MS.
Main Methods:
- Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) was employed.
- The study focused on noncovalent complexes (NCXs) involving peptides and proteins.
- Analysis was performed directly within a MALDI sample spot.
Main Results:
- MALDI-IMS successfully visualized the location and formation of noncovalent complexes (NCXs) in peptide and protein samples.
- The spatial distribution of NCXs within the MALDI sample spot was mapped.
- The study provided insights into the formation dynamics of these complexes under MALDI conditions.
Conclusions:
- MALDI imaging mass spectrometry is a valuable tool for spatially resolving noncovalent complexes (NCXs) in complex biological samples.
- This technique offers a unique perspective on the formation and distribution of peptide and protein NCXs.
- Further optimization of experimental parameters can enhance the analysis of NCXs using MALDI-IMS.
More Related Videos
Related Concept Videos
01:19MALDI-TOF Mass Spectrometry
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
01:21Tandem Mass Spectrometry
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
01:33Peptide Identification Using Tandem Mass Spectrometry
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
01:08Matrix-Assisted Laser Desorption Ionization (MALDI)
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
01:21Mass Spectrometry: Complex Analysis
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
01:19Mass Spectrometry: Overview
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...