Hematologic reference intervals in Cynomolgus (Macaca fascicularis) monkeys.

Area of Science:

• Comparative Pathology
• Primate Hematology
• Biomedical Research

Background:

• Cynomolgus monkeys (Macaca fascicularis) are crucial non-human primate models in biomedical research.
• Accurate interpretation of clinical pathology data relies on established reference intervals.
• Existing reference intervals may not fully represent diverse Cynomolgus populations.

Purpose of the Study:

• To establish robust hematologic reference intervals for Cynomolgus monkeys.
• To adhere to guidelines set by the International Federation of Clinical Chemistry and Clinical and Laboratory Standards Institute.
• To provide a reliable data set for primatology research centers.

Main Methods:

• Analysis of blood specimens from 272 healthy Cynomolgus monkeys.
• Utilized a nonparametric method for reference interval establishment.
• Investigated the influence of age, sex, body weight, and geographic origin on hematologic parameters.

Main Results:

• Age-related trends observed: slight decreases in hemoglobin, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration, with a slight increase in mean corpuscular volume.
• Geographic origin impacts: monkeys from the Philippines exhibited lower red blood cell concentration, hemoglobin, and hematocrit.
• Established reference intervals provide a benchmark for hematologic parameters in this species.

Conclusions:

• The established hematology reference intervals align with international recommendations.
• These intervals are applicable to research settings utilizing similar Cynomolgus monkey populations and hematology analyzers.
• This study contributes to standardized data interpretation in non-human primate research.