Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

919
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
919
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.4K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane Protrusions.

Research square·2026
Same author

Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane Protrusions.

bioRxiv : the preprint server for biology·2026
Same author

Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy.

bioRxiv : the preprint server for biology·2026
Same author

Multi-immersion Oblique Plane Microscope (miOPM): A reconfigurable platform for high-resolution Light-Sheet Fluorescence Microscopy.

bioRxiv : the preprint server for biology·2025
Same author

Universal consensus 3D segmentation of cells from 2D segmented stacks.

Nature methods·2025
Same author

Combinatorial responsiveness of chemosensory neurons in mouse explants revealed by DynamicNeuroTracker.

Cell reports methods·2025
Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026
Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026
Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026
Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026
Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026
See all related articles

Related Experiment Video

Updated: May 7, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

27.0K

Three-beam interference with circular polarization for structured illumination microscopy.

Hsiao-Chih Huang, Bo-Jui Chang, Li-Jun Chou

    Optics Express
    |October 10, 2013
    PubMed
    Summary
    This summary is machine-generated.

    A simplified three-dimensional structured illumination microscopy (3D-SIM) method uses circular polarization for consistent high-contrast imaging. This technique enhances resolution and image quality in microscopy without complex equipment.

    More Related Videos

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    10.0K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    8.7K

    Related Experiment Videos

    Last Updated: May 7, 2026

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    27.0K
    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    10.0K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    8.7K

    Area of Science:

    • Optical Microscopy
    • Super-resolution Imaging
    • Biophysics

    Background:

    • Three-dimensional structured illumination microscopy (3D-SIM) enhances resolution and contrast in wide-field microscopy.
    • Traditional 3D-SIM methods often require complex polarization control or mechanical components to maintain modulation contrast.
    • Achieving consistent modulation contrast across all orientations is crucial for reliable 3D-SIM imaging.

    Purpose of the Study:

    • To demonstrate a simplified approach for generating structured illumination patterns in 3D-SIM.
    • To achieve consistent modulation contrast in 3D-SIM imaging independent of orientation.
    • To facilitate the implementation and broaden the application of 3D-SIM systems.

    Main Methods:

    • Utilized three-beam interference with circular polarization to generate the structured illumination pattern.
    • Derived the intensity distribution of the interference pattern to confirm orientation-independent modulation contrast.
    • Compared simulated SIM images of 100-nm beads using linear versus circular polarization and validated with experimental results.

    Main Results:

    • Demonstrated that circular polarization in three-beam interference maintains consistent modulation contrast across all orientations.
    • Simulations showed the influence of modulation contrast on 3D-SIM imaging of nanoparticles.
    • Experimental results confirmed the simulation findings, validating the simplified approach.

    Conclusions:

    • A simple method using a quarter-wave plate to convert linear to circular polarization enables orientation-independent modulation contrast in 3D-SIM.
    • This simplified 3D-SIM approach, combined with a spatial light modulator, reduces system complexity.
    • The technique is expected to enhance the accessibility and application range of 3D-SIM.