Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.4K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.8K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Choroid plexus remodeling linked to impaired CSF-mediated clearance and Alzheimer's disease progression.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Preoperative urge urinary incontinence and outcomes after thulium laser enucleation for benign prostatic hyperplasia.

World journal of urology·2026
Same author

Refractory Malignant Arrhythmia in a 4-Year-Old Child With Short QT Syndrome: Persistence for Hope.

JACC. Case reports·2026
Same author

Photorealistic 3D Holographic Display with Natural Defocus Effect.

Nature communications·2026
Same author

Phase contrast tomography (PCT).

Optics express·2026
Same author

Analysis of Clavien-Dindo classification and risk factors for complications after transurethral Thulium Laser enucleation of the prostate.

Medicine·2026
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: May 7, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.1K

Noninterferometric single-shot quantitative phase microscopy.

Chao Zuo, Qian Chen, Weijuan Qu

    Optics Letters
    |October 10, 2013
    PubMed
    Summary
    This summary is machine-generated.

    We developed a fast, single-shot quantitative phase microscopy method using the transport of intensity equation (TIE). This technique accurately measures cellular dynamics and biological processes in real-time.

    More Related Videos

    Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
    07:38

    Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

    Published on: September 13, 2016

    8.0K
    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
    10:20

    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

    Published on: September 5, 2019

    7.9K

    Related Experiment Videos

    Last Updated: May 7, 2026

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
    14:09

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

    15.1K
    Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
    07:38

    Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

    Published on: September 13, 2016

    8.0K
    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
    10:20

    Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

    Published on: September 5, 2019

    7.9K

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Cell Biology

    Background:

    • Quantitative phase microscopy (QPM) is crucial for label-free cell imaging.
    • Traditional QPM methods often require complex setups or multiple measurements, limiting dynamic studies.
    • The transport of intensity equation (TIE) offers a path to phase retrieval from intensity images.

    Purpose of the Study:

    • To introduce a noninterferometric, single-shot QPM technique.
    • To enable high-speed, camera-limited phase recovery for dynamic cellular processes.
    • To validate the method's accuracy and explore its biological applications.

    Main Methods:

    • A Michelson-like optical configuration integrated with a standard inverted microscope.
    • Simultaneous acquisition of two laterally separated images from different focal planes in a single exposure.
    • Application of the transport of intensity equation (TIE) for phase reconstruction.
    • Validation using precise measurements of a microlens array.

    Main Results:

    • Demonstrated accurate phase recovery from single-shot, dual-plane intensity images.
    • Achieved camera-limited frame rates for phase imaging.
    • Successfully applied the technique to study chemical-induced apoptosis.
    • Visualized the phagocytosis process in macrophages with high temporal resolution.

    Conclusions:

    • The developed single-shot TIE-based QPM is accurate and efficient.
    • The technique facilitates dynamic studies of cellular processes like apoptosis and phagocytosis.
    • This method holds significant promise for advanced live-cell imaging and biological research.